Manual threshold

Binarises an image (or image stack) using a fixed intensity threshold.

Description

Binarises an image (or image stack) using a fixed intensity threshold. The input threshold can be a single value (same for all images) or taken from a measurement associated with the image to be binarised.

Parameters

ParameterDescription
Input imageImage to apply threshold to.
Apply to input imageSelect if the threshold should be applied directly to the input image, or if it should be applied to a duplicate, then stored as a different image in the workspace.
Output imageName of the output image created during the thresholding process. This image will be added to the workspace.
Threshold sourceSource for the threshold value:
  • "Fixed value" Uses a single, fixed value for all images. This value is specified using the "Threshold value" parameter.
  • "Image measurement" Threshold is set to the value of a measurement assoiated with the image to be binarised. Measurement selected by "Measurement" parameter. In this mode a different threshold can be applied to each image.
Threshold valueAbsolute manual threshold value that will be applied to all pixels.
MeasurementMeasurement to act as threshold value when in "Image measurement" mode.
Binary logicControls whether objects are considered to be white (255 intensity) on a black (0 intensity) background, or black on a white background.