Manual threshold

Binarises an image (or image stack) using a fixed intensity threshold.

Description

Binarises an image (or image stack) using a fixed intensity threshold. The input threshold can be a single value (same for all images) or taken from a measurement associated with the image to be binarised.

Parameters

Parameter Description
Input image Image to apply threshold to.
Apply to input image Select if the threshold should be applied directly to the input image, or if it should be applied to a duplicate, then stored as a different image in the workspace.
Output image Name of the output image created during the thresholding process. This image will be added to the workspace.
Threshold source Source for the threshold value:
  • "Fixed value" Uses a single, fixed value for all images. This value is specified using the "Threshold value" parameter.
  • "Image measurement" Threshold is set to the value of a measurement assoiated with the image to be binarised. Measurement selected by "Measurement" parameter. In this mode a different threshold can be applied to each image.
Threshold value Absolute manual threshold value that will be applied to all pixels.
Measurement Measurement to act as threshold value when in "Image measurement" mode.
Binary logic Controls whether objects are considered to be white (255 intensity) on a black (0 intensity) background, or black on a white background.